Sophie Bernard, Glycomev EA4358

Fonction/Function : : Ingénieur d’étude en Microscopie électronique à transmission / Transmission electron microscopy engineer

Adresse/Address : Laboratoire « Glycobiologie et Matrice Extracellulaire Végétale », Glyco-MEV, Université de Rouen 76821 Mont-Saint-Aignan, France

2009-Present : Transmission electron microscopy engineer in the Research Laboratory « Glycobiology and plant extracellular matrix » and the Imaging platform PRIMACEN, Rouen University.
2008-2009 : Non permanent Teacher and Researcher position, Rouen University.

2005-2008 : PhD student in cellular biology in the academic research laboratory “Glycobiology and extracellular matrix”, EA4358, Rouen University. Project: “Mapping cell wall synthesizing enzymes within plant Golgi cisternae using TEM and enzymatic complexes research”
Six months PhD training in the academic research laboratory “Plant cell wall biotechnology: cell wall elaboration”, Ohio university (Athens,OH), USA and two months PhD training in the academic research laboratory “Molecular plant biology: Cell wall”, University of Copenhagen, Denmark.

2004-2005 : Master degree in Cellular Biology, speciality « Ecophysiology and plant biotechnology ».

- Sample type: Nicotiana tabacum and Arabidopsis thaliana plants (tissue or suspension-cultured cells), cosmetic cream, hydrogel, biopolymer.
- Sample preparation techniques: chemical fixation, high pressure freezing, freeze substitution, embedding in LRW or Spurr resin.
- Ultramicrotomy: semi-fine and fine sections.

Follet-Gueye ML, Mollet JC, Vicré-Gibouin M, Bernard S, Chevalier L, Plancot B, Dardelle F, Ramdani Y, Coimbra S, Driouich A (2012) Immuno-Glyco-imaging in plant cells: localization of cell wall carbohydrate epitopes and their biosynthesizing enzymes. In: Dehghani H (ed) Immunocytochemistry, InTech Publisher. pp. 297-320. ISBN 978-953-51-0229-8.

Elias S, Delestre C, Ory S, Marais S, Courel M, Vazquez-Martinez R, Bernard S, Coquet L, Malagon MM, Driouich A, Chan P, Gasman S, Anouar Y, Montero-Hadjadje M. (2012) Chromogranin a induces the biogenesis of granules with calcium- and actin-dependent dynamics and exocytosis in constitutively secreting cells. Endocrinology, 153:4444-4456.
Driouich A, Follet-Gueye ML, Bernard S, Kousar S, Chevalier L, Vicré-Gibouin M, Lerouxel O. (2012) Golgi-mediated synthesis and secretion of matrix polysaccharides of the primary cell wall of higher plants. Frontiers in Plant Science, doi: 10.3389/fpls.2012.00079.
C.Søgaard, S. Bernard, M. Hadi, A. Driouich, HV. Scheller,Y. Sakuragi. (2012) PROMTO illuminates protein membrane topology in the Golgi apparatus of living tissue. PloS One, 7:e31324.
Harholt J, Jensen JK, Verhertbruggen Y, Søgaard C, Bernard S, Nafisi M, Poulsen CP, Geshi N, Sakuragi Y, Driouich A, Knox JP, Scheller HV. (2012) ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta. Planta, DOI: 10.1007/s00425-012-1592-3.
Chevalier L, Bernard S, Driouich A and Follet-Gueye ML. (2011) Cryopreparation of tobacco Bright Yellow (BY-2) suspension-cultured cells and application to immunogold localization of Green Fluorescent Protein-tagged-membrane- proteins. Journal of biological research-Thessaloniki, 16, 202-216.
Chevalier L*, Bernard S*, Ramdani Y, Lamour R, Bardor M, Lerouge P, Follet-Gueye ML, Driouich A. (2010) Subcompartment localization of the side chain xyloglucan-synthesizing enzymes within Golgi stacks of tobacco suspension-cultured cells. Plant Journal (64)6:977-89
Wu Y, Williams M, Bernard S, Driouich A, Showalter AM, Faik A. (2010) Functional identification of two nonredundant Arabidopsis alpha(1,2)fucosyltransferases specific to arabinogalactan proteins. Journal of biological chemistry 285(18): 13638-45