Fonction/Function : Maître de Conférences / Associate Professor
Tel : 33 (0)2 35 14 67 58
e-mail : muriel.bardor@univ-rouen.fr
Adresse/Address : Laboratoire « Glycobiologie et Matrice Extracellulaire Végétale », Glyco-MEV, Université de Rouen 76821 Mont-Saint-Aignan, France
Academic Qualifications and Career :
1998: DEA de Biologie cellulaire, Université de Rouen / Master's degree in Cell Biology, University of Rouen, France.
2001: Doctorat européen en Biochimi, Université de Rouen / European PhD in Biochemistry, University of Roue, France.
2009 : Diplôme d'Habilitation à Diriger des recherches, Université de Rouen (French University Qualification to Supervise PhD students).
Academic Qualifications and Career :
2002-2003 : Checrheur post-doctorant / Post-doctoral research associate, Pr. Ajit Varki's, Glycobiology Research and Training Center, University of California San Diego, USA.
2003-present : Maître de Conférences en Biochimie / Associate Professor in Biochemistry, University of Rouen, France.
2010-2012: Détachement à l’étranger / oversea long-period stay as a Research scientist in the Analytics group at Bioprocessing Technology Institute, Singapore
Other professional activities
2003-present : Teaching in Biochemistry, Cell Biology, Plant Biotechnology (192h/year) 2003-present : Administrative responsibilities and organization of teaching at the university
2004-present : Reviewer for different international journals
Zhang P., Chan K.F., Haryadi R., Bardor M. and Song Z. (2012) CHO Glycosylation Mutants as Potential Host Cells to Produce Therapeutic Proteins with Enhanced Efficacy. Adv Biochem Eng Biotechnol. In press.
Mueller M., Wan C., Hoi K.M., Kim D.Y., Gan H.T., Bardor M.* and Gagnon P.* (2012) Immunoglobulins M survive low pH conditions used for virus inactivation and for elution from bioaffinity columns." the Journal of Pharmaceutical Sciences, In press. IF 3.055. *Equal contributors of the senior authors.
Ng S.K., Tan T.R.M., Wang Y., Ng D., Goh L.-T., Bardor M., Wong V.V.T. and Lam K.P. (2012) Production of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector. PloS ONE, 7(12):e52785. IF 4.1.
Mueller M., Loh M.Q., Tscheliessnig R., Tee D.H., Tan E., Bardor M. and Jungbauer A. (2012) Liquid Formulations for Stabilizing IgMs During Physical Stress and Long-Term Storage. Pharmaceutical Research, In press. IF 4.4.
van Beers M. and Bardor M. (2012) Minimizing immunogenicity of biopharmaceuticals by controlling critical quality attributes of proteins. Biotechnology Journal, 12, 1473-1484. IF 2.6.
Lingg N.*, Zhang P.*, Song Z. and Bardor M. (2012) The sweet tooth of biopharmaceuticals: Importance and analysis of recombinant protein glycosylation. Biotechnology Journal., 12, 1462-1472. IF 2.6. *These authors have equally contributed to the work.
Ho S.C.L., Bardor M., Feng H., Mariati, Hosokai Y., Song Z., Tong Y.W., Yap M.G.S. and Yang Y.S. (2012) An optimized IRES-mediated tricistronic vector for rapid generation of stable, high monoclonal antibody expressing CHO cell lines. Journal of Biotechnology, 157, 130-9. IF: 3.
Baiet B, Burel C, Saint-Jean B, Louvet R, Menu-Bouaouiche L, Kiefer-Meyer MC, Mathieu-Rivet E, Lefebvre T, Castel H, Carlier A, Cadoret JP, Lerouge P, Bardor M. (2011) N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme. Journal of Biological Chemistry, 286(8):6152-64.
Louvet R, Rayon C, Domon JM, Rusterucci C, Fournet F, Leaustic A, Crépeau MJ, Ralet MC, Rihouey C, Bardor M, Lerouge P, Gillet F, Pelloux J. (2011) Major changes in cell wall during silique development in Arabidopsis thaliana. Phytochemistry, 72, 59-67.
Chevalier L, Bernard S, Ramdani Y, Lamour R, Bardor M, Lerouge P, Follet-Gueye ML, Driouich A. (2010) Subcompartiment localization of two xyloglucan-synthesizing enzymes within the Golgi stacks of tobacco suspension-cultured cells. Plant Journal, 64, 977-989.
Dardelle F, Lehner A, Ramdani Y, Bardor M, Lerouge P, Driouich A, Mollet JC. (2010) Biochemical and immunocytological characterizations of Arabidopsis thaliana pollen tube cell wall. Plant Physiology, 153, 1563-1576.
Triguero A, Cabrera G, Royle L, Harvey DJ, Rudd PM, Dwek RA, Bardor M, Lerouge P, Cremata JA (2010) Chemical and enzymatic N-glycan release comparison for N-glycan profiling of monoclonal antibodies expressed in plants. Analytical Biochemistry 400: 173-183.
Bardor M, Cabrera G, Stadlmann J, Lerouge P, Cremata JA, Gomord V, Fitchette AC. (2009). N-glycosylation of plant recombinant pharmaceuticals. Methods in Molecular Biology 483: 239-264.
Benchabane M, Saint-Jore-Dupas C, Bardor M, Faye L, Michaud D, Gomord V (2009) Targeting and post-translational processing of human alpha(1)-antichymotrypsin in BY-2 tobacco cultured cells dagger. Plant Biotechnology Journal 7: 146-160
Aboughe-Angone S, Bardor M, Nguema-Ona E, Rihouey C, Ishii T, Lerouge P, Driouich A (2009) Structural characterization of cell wall polysaccharides from two plant species endemic to central Africa, Fleurya aestuans and Phragmenthera capitata. Carbohydrate Polymers 75: 104-109.
Vezina LP, Faye L, Lerouge P , D'Aoust MA , Marquet-Blouin E , Burel C , Lavoie PO, Bardor M, Gomord V (2009) Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants. Plant Biotechnology Journal 7: 442-455.
Sourrouille C, Marquet-Blouin E, D'Aoust MA, et al. (2008) Down-regulated expression of plant-specific glycoepitopes in alfalfa. Plant Biotechnology Journal 6, 702-721.
Seveno M, Cabrera G, Triguero A, et al. (2008) Plant N-glycan profiling of minute amounts of material. Analytical Biochemistry 379, 66-72.
Cadoret JP, Bardor M, Lerouge P, et al. (2008) Producing recombinant commercial proteins. M S-Medecine Sciences 24, 375-382.
Bosco M, Le Gall S, Rihouey C, et al. (2008) 6-AzidO D-galactose transfer to N-acetyl-D-glucosamine derivative using commercially available beta-1,4-galactosyltransferase. Tetrahedron Letters 49, 2294-2297.
Loutelier-Bourhis C, Bardor M, Lange CM (2008) Structural characterization of trimethylsilyl methyl glycosides derivatives by high-resolution mass spectrometry, linked scans and MIKE experiments. Analytical and Bioanalytical chemistry 390, 1853-1860.
Lienard D, Dinh OT, van Oort E, et al. (2007) Suspension-cultured BY-2 tobacco cells produce and mature immunologically active house dust mite allergens. Plant Biotechnology Journal 5, 93-108. 2007
Paccalet T, Bardor M, Rihouey C, et al. (2007) Engineering of a sialic acid synthesis pathway in transgenic plants by expression of bacterial Neu5Ac-synthesizing enzymes. Plant Biotechnology Journal 5, 16-25.
Bardor M, Cabrera G, Rudd P, Cremata J, Lerouge P (2006) Analytical strategies to investigate plant N-glycan profiles in the context of plant-made pharmaceuticals. CurrentOpinion in Structural Biology 16, 576-583.
Nguema-Ona E, Andeme-Onzighi C, Aboughe-Angone S, et al. (2006) The reb1-1 mutation of Arabidopsis. Effect on the structure and localization of galactose-containing cell wall polysaccharides. Plant Physiology 140, 1406-1417.
Petruccelli S, Otegui MS, Lareu F, et al. (2006) A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds. Plant Biotechnology Journal 4, 511-527.
Bardor M, Nguyen DH, Diaz S, Varki A (2005) Mechanism of uptake and incorporation of the non-human sialic acid N-glycolylneuraminic acid into human cells. Journal of Biological Chemistry 280, 4228-4237.
Séveno M, Bardor M, Paccalet T, Gomord V, Lerouge P, Faye L (2004) Glycoprotein sialylation in plants? Nature Biotechnology 22, 5-6.
Sriraman R, Bardor M, Sack M, et al. (2004) Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core-alpha(1,3)-fucose residues. Plant Biotechnology Journal 2, 279-287.
Gomord W, Sourrouille C, Fitchette AC, et al. (2004) Production and glycosylation of plant-made pharmaceuticals: the antibodies as a challenge. Plant Biotechnology Journal 2, 83-100.
Bardor M, Faveeuw, Fitchette, Gilbert D, Galas L, Trottein F, Faye L,Lerouge P (2003). Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha(1,3) fucose and core xylose. Glycobiology 13, 427-434.
Bardor M, Loutelier-Bourhis C, Paccalet T, Cosette P, Fitchette AC, Vezina L, Trepanier S, Dargis M, Lemieux R, Lange C, Faye L, Lerouge P (2003) Monoclonal C5-1 antibody produced in alfalfa plants exhibits a N-glycosylation that is homogenous and suitable for glyco-engineering into human-compatible structures. Plant Biotechnology Journal 1, 451-462.
Tangvoranuntakul P, Gagneux P, Diaz S, et al. (2003) Human uptake and incorporation of an immunogenic nonhuman dietary sialic acid. Proceedings of the National Academy of Sciences of the United States of America 100, 12045-12050.
Elbers IJW, Stoopen GM, Bakker H, et al. (2001) Influence of growth conditions and developmental stage on N-glycan heterogeneity of transgenic immunoglobulin G and endogenous proteins in tobacco leaves. Plant Physiology 126, 1314-1322.
Bakker H, Bardor M, Molthoff JW, et al. (2001) Galactose-extended glycans of antibodies produced by transgenic plants.Proceedings of the National Academy of Sciences of the United States of America98, 2899-2904.
Lemoine J, Cabanes-Macheteau M, Bardor M, et al. (2000) Analysis of 8-aminonaphthalene-1,3,6-trisulfonic acid labelled N-glycans by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 14, 100-104.
Bardor M, Faye L, Lerouge P (1999) Analysis of the N-glycosylation of recombinant glycoproteins produced in transgenic plants. Trends in Plant Science 4, 376-380.
Bardor M, Loutelier-Bourhis C, Marvin L, et al. (1999) Analysis of plant glycoproteins by matrix-assisted laser desorption ionisation mass spectrometry: Application to the N-glycosylation analysis of bean phytohemagglutinin. Plant Physiology and Biochemistry 37, 319-325.
