Fonction/Function : chercheur post-doctorant / Post-doctoral research associate
Tel : 33 (0)2 35 14 60 43
e-mail : eric.nguema-ona@univ-rouen.fr
Adresse/Address : Laboratoire « Glycobiologie et Matrice Extracellulaire Végétale », Glyco-MEV, Université de Rouen 76821 Mont-Saint-Aignan, France
Academic Qualifications and Career :
2003-2007: Doctorat en Biologie Cellulaire Végétale / PhD in Plant Cell Biology, Université de Rouen, France 2007-2008: Attaché Temporaire d’Enseignement et de Recherche / Non permanent Teacher and Researcher position, Université de Rouen, France. 2008-2011: Chercheur post-doctorant / Post-doctoral research associate, “Institute for Wine Biotechnology, faculty of Agrisciences”, Stellenbosch university, South Africa 2011-Present : Chargé de recherche (postdoctorant), Laboratoire « Glycobiologie et Matrice Extracellulaire Végétale », Glyco-MEV, Université de Rouen 76821 Mont-Saint-Aignan, France
Light Microscopy techniques
Immunochemistry coupled to Fluorescence and Electron microscopy: Immunolocalisation of carbohydrates and glycoproteins associated epitopes in plants.
Laser scanning confocal microscopy : Live cell imaging of cell wall protein tagged with GFP reporter.
Biochemistry and Analytical Chemistryfor Polysaccharides and glycoproteins
Chromatography: High pressure Liquid (HPLC/ HPAEC), Gas Chromatography (GC)-Flame ionisation detection (FID), Gas Chromatography (GC) coupled to mass spectrometer detection (GC-MSD) Mass Spectrometry: Maldi-Tof-MS, ESI-MS
Electrophoresis: SDS, PAGE,
Principales publications / Main publications
Nguema-Ona E, Vicré-Gibouin M, Cannesan MA, Driouich A. 2013. Arabinogalactan proteins in root–microbe interactions. Trends in Plant Science, doi:pii: S1360-1385(13)00059-9. 10.1016/j.tplants.2013.03.006.
Eric Nguema-Ona, John P Moore, Alexandra D Fagerstrom, Jonatan U Fangel, William GT Willats, Annatjie Hugo and Melané A Vivier (2013) Overexpression of the grapevine PGIP1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection. BMC Plant Biology, 13:46.
Moore JP, Nguema-Ona E, Vicre-Gibouin M, Sørensen I, Willats WGT, Driouich A, Farrant JM. (2012) Arabinose-rich polymers as an evolutionary strategy to plasticize resurrection plant cell walls against desiccation. Planta, 237 :739-754.
Nguema-Ona E, Coimbra S, Vicré-Gibouin M, Mollet JC, Driouich A. (2012) Arabinogalactan proteins in root and pollen tube cells: distribution and functional aspects. Annals of Botany, 110: 383-404.
Eric Nguema-Ona, John P. Moore, Alexandra Fagerström, Jonatan U. Fangel, William G.T. Willats,
Annatjie Hugo, Melané A. Vivier (2012) Profiling the main cell wall polysaccharides of tobacco leaves using
high-throughput and fractionation techniques. Carbohydrate polymers 88: 939-949.
Erik Alexandersson, John V.W. Becker, Dan Jacobson, Eric Nguema-Ona, Cobus Steyn, Katherine J. Denby and Melané A. Vivier (2011) Constitutive expression of a grapevine VvPGIP1 affects gene expression globally and influences functions related to the cell wall in transgenic tobacco. BMC Research Notes4:493.
Sophie Aboughe-Angone, Eric Nguema-Ona, Thaddée Boudjeko and Azeddine Driouich (2011) Plant cell wall polysaccharides: immmunomodulators of the immune system and source of natural fibers.Current topics in phytochemistry, 10:1-16.
Njintang Yanou Nicolas, Boudjeko Thaddée, Scher Joël, Nguema-Ona Eric and Mbofung Carl M.F. (2011) Monosaccharides and amino acids compositions of the mucilage of some varieties of taro (Colocasia esculentaL. Schott) corms. Journal of food Science and Technology doi 10.1007/s13197-011-0580-0.
Sophie Aboughe-Angone,Eric Nguema-Ona, Partha Ghosh, Patrice Lerouge, Tadashi Ishii, Bimalendu Ray and Azeddine Driouich (2008) Cell wall polysaccharides from fruit pulp of Argania spinosa: structural analysis of of pectin and xyloglucan polysaccharidesCarbohydrate research343: 67-72.
Sophie Aboughe-Angone, Muriel Bardor, Eric Nguema-Ona, Christophe Rihouey, Tadashi Ishii, Patrice Lerouge and Azeddine Driouich (2009) Structural characterization of cell wall polysaccharides from two plant species endemic to central Africa, Fleurya aestuans and Phragmenthera capitata Carbohydrate polymers 75 (1): 104-109.
Eric Nguema-Ona, Alex Bannigan, Laurence Chevalier, Tobias I. Baskin, Azeddine Driouich (2007) Disruption of arabinogalactan proteins disorganizes cortical microtubules in the root of Arabidopsis thaliana. The Plant Journal 52: 240-251.
Eric Nguema-Ona, Christine Andème-Onzighi, Sophie Aboughe-Angone, Muriel Bardor, Tadashi Ishii, Patrice Lerouge and Azeddine Driouich (2006) The reb1-1 Mutation of Arabidopsis. Effect on the Structure and Localization of Galactose-Containing Cell Wall Polysaccharides. Plant Physiology 140: 1406-1417.
John P. Moore, Eric Nguema-Ona, Laurence Chevalier, George G. Lindsey, Wolf F. Brandt, Patrice Lerouge, Jill M. Farrant, and Azeddine Driouich (2006) Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius. Plant Physiology 141: 651-662.